EBTT 2020
Archived edition · Online

EBTT 2020

Electroporation-Based Technologies and Treatments — International Scientific Workshop & Postgraduate Course

16 November – 4 December 2020ONLINE event, Ljubljana, Slovenia5 ECTS · 29.5 CME

The meeting

Cell membrane electroporation — mechanisms & applications

Cell membrane electroporation, its mechanisms and applications in biology, medicine and biotechnology. The basis of the phenomena, current applications and future prospects were the main topics of this international meeting.

The postgraduate course was intended both for novices and experts in electroporation, including PhD students, researchers and users. Abstract submission was strongly encouraged and was necessary if credits (5 ECTS) were sought — each participant submitting an abstract also gave a short oral presentation of their work. The course was accredited by the Medical Chamber of Slovenia, so participants received 29.5 CME credits.

In the light of the global crisis and many difficulties caused by the Covid-19 pandemic, and after careful consideration of all available options, EBTT 2020 took place between 16 November and 4 December 2020 as an ONLINE event only. An online participation fee was introduced at registration.

Organised by the University of Ljubljana and the Institute of Oncology, Ljubljana. Read all about EBTT in an article published by MediaResearch Ltd, 2012.

Course content

Preliminary programme & topics

Electrical properties of cells & tissues

Resting & induced transmembrane voltage; dielectric properties of tissues; temperature & conductivity changes.

Physical chemistry of electroporation

Electrochemical membrane thermodynamics; molecular dynamics simulations.

Electroporation in vitro & in vivo

Protocols for cells in vitro and in vivo.

Devices & electrodes

Devices & electrodes for in vitro/in vivo work; tissue models of electroporation and their validation.

Electrochemotherapy of tumours

Basic mechanisms; preclinical studies; clinical use; current developments & future perspectives.

Gene transfection

Gene transfection in vitro/in vivo; electroporation for gene therapy and DNA vaccines.

Highlights of 2020

Bonus lectures of 2019

Faculty

Directors & lecturers

Co-DirectorDamijan Miklavčič
Co-DirectorLluis M. Mir
In memoriam: Justin Teissié (1947–2020)
Justin Teissié was a devoted scientist and a founding — and still active — faculty member of the EBTT. He dedicated his life to the study of biological membranes and the fundamental processes leading to membrane permeabilisation, and played a major role in the development of its applications. For his work he received many honours: winner of the Prize for Medical Applications of Electricity awarded by the Institut Électricité Santé in 1995, CNRS honorary medallist in 2013, and laureate of the American Institute for Medical and Biological Engineering (AIMBE) in 2017. He became an Honorary Senator of the University of Ljubljana in 2015, and was Emeritus Research Director from 2012. His passing creates an immense void in our community. We, his students, colleagues and friends, will never forget him.
— Marie-Pierre Rols and Damijan Miklavčič

Faculty members & lecturers

  • Damijan MiklavčičUniversity of Ljubljana, Slovenia
  • Lluis M. MirCNRS & Institut Gustave Roussy, Villejuif, France
  • Marie-Pierre RolsCNRS & IPBS, Toulouse, France
  • Gregor SeršaInstitute of Oncology, Ljubljana, Slovenia
  • Mounir TarekUniversity of Lorraine, Nancy, France
  • P. Thomas VernierOld Dominion University, Norfolk, Virginia, USA
  • Julie GehlUniversity of Copenhagen, Denmark

Invited lecturers

  • François H. CornelisSorbonne Université, Hôpital Tenon, Paris, France
  • Gale Louise CravisoUniversity of Nevada, Reno, Nevada, USA
  • Maja ČemažarInstitute of Oncology, Ljubljana, Slovenia
  • Tadej KotnikUniversity of Ljubljana, Slovenia
  • Indrawati OeyUniversity of Otago, Dunedin, New Zealand
  • Emanuela SignoriCNR – Institute of Translational Pharmacology & University of Roma Campus Bio-Medico, School of Medicine, Roma, Italy

Founding Faculty members

Organising committee

Programme & schedule

Schedule downloads

EBTT 2020 was organised as an online event from 16–27 November. Each weekday from 14:30 to 16:00 C.E.T. there were online lectures with live discussions; interactive practical-work sessions were set in different time slots to accommodate different time zones.

PDFFinal Schedule

Full programme (last update 20.11.2020)

 PDFPractical Work Schedule

Laboratory session timetable

 PDFShort Presentations Programme

Participants' short oral presentations (last update 18.11.2020)

 PDFInstructions for Virtual Platform

How to join the online sessions

Hands-on

Practical work & exercises

This year's practical lab works were organised as interactive online training sessions. Each session required active participation and was accompanied by various tasks. Active participation and completion of tasks of 3 practical work sessions were mandatory for obtaining ECTS and/or CME credits. Exercise sheets (PDF) as distributed to participants.

L1The influence of Mg2+ ions on gene electrotransfer efficiencyPDF ↓
L3Visualisation of local ablation zone distribution between two needle electrodesPDF ↓
L6Electroporation of planar lipid bilayersPDF ↓
L7E. coli inactivation by pulsed electric fields in a continuous flow systemPDF ↓
L8Analysis of electric field orientations on gene electrotransfer efficiencyPDF ↓
L10Monitoring of electric field distribution in biological tissue by means of magnetic resonance electrical impedance tomographyPDF ↓
L11Measurements of the induced transmembrane voltage with fluorescent dye di-8-ANEPPSPDF ↓
C1Treatment planning for electrochemotherapy and irreversible electroporation: optimization of voltage & electrode positionPDF ↓
C2Numerical modelling of thermal effects during irreversible electroporation treatmentsPDF ↓
C3Molecular dynamics simulations of membrane electroporationPDF ↓
E1Electroporation of cells and tissues — interactive e-learningPDF ↓

Abstracts & publication

Abstract submission

Participants submitted an abstract as PDF and MS Word file. For abstract writing the enclosed MS Word template was used.

PDFAbstract Submission Form PDFInstructions for Abstract Submission DOCAbstract Template

See also the general Journal of Membrane Biology author guidelines and the workshop guidance for lectures.

Proceedings

Electronic proceedings

EBTT 2020 proceedings cover

Electronic proceedings — all abstracts and course material of EBTT 2020.

Download proceedings (PDF)

Support

Supported by

Educell Energy Pulse Systems IGEA Iskra Medical Laboratorium Medtronic mikro+polo Omega Pulse Biosciences

Contacts

Organisation & contacts

Registration, abstract submission & other

Damijan Miklavčič
Faculty of Electrical Engineering, University of Ljubljana
Tržaška 25, 1000 Ljubljana, Slovenia
damijan.miklavcic@fe.uni-lj.si
tel +386 1 4768 456

Accommodation & travel arrangements

Congress Agency ANTANA PCO
Mrs. Nina Bernard
nina.bernard@antana-pco.com
tel +386 41 229 222

The EBTT series

Previous & other editions

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