November 16 - December 4, 2020

ONLINE Event, Ljubljana, Slovenia

Electroporation-Based Technologies and Treatments



Get the book

Preliminary programme:

Electrical properties of cells and tissues in electric field

  • Resting and induced transmembrane voltage
  • Dielectric properties of biological tissues
  • Temperature and conductivity changes in tissues
  • Physical chemistry of membrane electroporation

  • Electrochemical membrane thermodynamics
  • Molecular dynamics simulations
  • Electroporation in vitro and in vivo - protocols

  • Electroporation of cells in vitro
  • Electroporation of cells in vivo
  • Development of devices and electrodes

  • Devices for in vitro and in vivo experimental work
  • Electrodes for in vitro and in vivo
  • Electroporation of cells in tissue - models of electroporation in vivo and their experimental validations
  • Electrochemotherapy of tumors

  • Basic mechanisms of electrochemotherapy
  • Preclinical studies
  • Clinical use of electrochemotherapy
  • Current developments and future perspectives
  • Application of electroporation in gene transfection

  • Gene transfection in vitro
  • Gene transfection in vivo
  • Electroporation for gene therapy
  • Electroporation for DNA vaccine
  • Highlights of 2020:

  • The applications of ECT/IRE in clinical practice, François H. Cornelis
  • Nanosecond electrical activation of adrenal chromaffin cells, Gale Craviso
  • Muscle contractions: avoiding them in electroporation treatments and inducing them with injectable microstimulators for movement restoration in paralysis, Toni Ivorra
  • Effect of Pulsed Electric Fields processing on plant-based foods, Indrawati Oey
  • Bonus lectures of 2019:

  • Electric fields and microfluidics for lab-on-chip applications, Jonathan Cottet
  • Effects of electroporation and electrochemotherapy on normal and tumor blood vessels, Boštjan Markelc
  • PEF treatment applications in food technology and limitations, Gianpiero Pataro
  • Multiscale modelling of electroporation, Lea Rems
  • Laboratory and practical work

  • The influence of Mg2+ ions on gene electotransfer efficiency
  • Monitoring cell membrane electropermeabilization with ratiometric fluorescence dye FURA-2AM
  • Electropermeabilization detection with propidium iodide in plated cells
  • Measurements of the induced transmembrane voltage with fluorescent dye di-8-ANEPPS
  • Electrotransformation of Escherichia coli with plasmid DNA
  • E. coli inactivation in a continuous flow system
  • Electroporation of planar lipid bilayers
  • Analysis of electric field orentations on gene electrotransfer visualization at the membrane level
  • Treatment planning for electrochemotherapy and irreversible electroporation: optimization of voltage and electrode position
  • Numerical Modeling of Thermal Effects during Irreversible Electroporation Treatments
  • Molecular dynamics simulations of membrane electroporation
  • Electroporation of cells and tissues - interactive e-learning
  • Measurement of electroporation pules with osciloscope; voltage and current probes
  • Development of pulse power generators for electroporation