International SCIENTIFIC WORKSHOP and POSTGRADUATE COURSE
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Preliminary programme:
Electrical properties of cells and tissues in electric field
Resting and induced transmembrane voltage
Dielectric properties of biological tissues
Temperature and conductivity changes in tissues
Physical chemistry of membrane electroporation
Electrochemical membrane thermodynamics
Molecular dynamics simulations
Electroporation in vitro and in vivo - protocols
Electroporation of cells in vitro
Electroporation of cells in vivo
Development of devices and electrodes
Devices for in vitro and in vivo experimental work
Electrodes for in vitro and in vivo
Electroporation of cells in tissue - models of electroporation in vivo and their experimental validations
Electrochemotherapy of tumors
Basic mechanisms of electrochemotherapy
Preclinical studies
Clinical use of electrochemotherapy
Current developments and future perspectives
Application of electroporation in gene transfection
Gene transfection in vitro
Gene transfection in vivo
Electroporation for gene therapy
Electroporation for DNA vaccine
Highlights of 2020:
The applications of ECT/IRE in clinical practice, François H. Cornelis
Nanosecond electrical activation of adrenal chromaffin cells, Gale Craviso
Muscle contractions: avoiding them in electroporation treatments and inducing them with injectable microstimulators for movement restoration in paralysis, Toni Ivorra
Effect of Pulsed Electric Fields processing on plant-based foods, Indrawati Oey
Bonus lectures of 2019:
Electric fields and microfluidics for lab-on-chip applications, Jonathan Cottet
Effects of electroporation and electrochemotherapy on normal and tumor blood vessels, Boštjan Markelc
PEF treatment applications in food technology and limitations, Gianpiero Pataro
Multiscale modelling of electroporation, Lea Rems
Laboratory and practical work
The influence of Mg2+ ions on gene electotransfer efficiency
Monitoring cell membrane electropermeabilization with ratiometric fluorescence dye FURA-2AM
Electropermeabilization detection with propidium iodide in plated cells
Measurements of the induced transmembrane voltage with fluorescent dye di-8-ANEPPS
Electrotransformation of Escherichia coli with plasmid DNA
E. coli inactivation in a continuous flow system
Electroporation of planar lipid bilayers
Analysis of electric field orentations on gene electrotransfer visualization at the membrane level
Treatment planning for electrochemotherapy and irreversible electroporation: optimization of voltage and electrode position
Numerical Modeling of Thermal Effects during Irreversible Electroporation Treatments
Molecular dynamics simulations of membrane electroporation
Electroporation of cells and tissues - interactive e-learning
Measurement of electroporation pules with osciloscope; voltage and current probes
Development of pulse power generators for electroporation
